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研究テーマ



研究課題:マイクロRNAによる顎下腺形態形成の制御機構研究課題:顎下腺形態形成に関する研究研究課題:耳下腺アミラーゼ分泌を基とした開口放出研究課題:準備中研究課題:外分泌低下モデルから紐解く外分泌メカニズム

研究業績

  • Shitara, A., Malec, I., Ebrahim, S., Chen, D., Bleck, C., Hoffman, M. P., Weigert, R.: Cdc42 negatively regulates endocytosis during apical membrane maintenance in live animals. Mol. Biol. Cell, doi:10.1091/mbc.E18-10-0615
  • Shitara, A., Malec, I., Ebrahim, S., Chen, D., Bleck, C., Hoffman, M. P., Weigert, R.: Cdc42 negatively regulates endocytosis during apical plasma membrane maintenance and development in mouse tubular organs in vivo. bioRxiv, doi:10.1101/402677
  • Satoh, K., Ouchi, M., Morita, A., Kashimata, M.: MARCKS phosphorylation and amylase release in GLP-1-stimulated acini isolated from rat pancreas. J. Physiol. Sci., doi: 10.1007/s12576-018-0621-9
  • Ohno, Y., Niwa, T., Hirai, K., Suzuki, K., Yamada, Y., Hayashi, Y., Hayashi, H., Suzuki, A., Itoh, Y.: Time-Dependent Decline in Serum Phenytoin Concentration with Heightened Convulsive Seizure Risk by Prolonged Administration of Fosphenytoin in Japanese: A Retrospective Study. Ther. Drug. Monit., doi: 10.1097/FTD.0000000000000521
  • Kashimata, M., Hayashi, T.: Regulatory mechanisms of branching morphogenesis in mouse submandibular gland rudiments. Jpn. Dent. Sci. Rev., 54, 2-7, 2018
  • Morita, A., Ouchi, M., Terada, M., Kon, H., Kishimoto, S., Satoh, K., Otani, N., Hayashi, K., Fujita, T., Inoue, K.I., and Anzai, N.: Reproducible insulin secretion from isolated rat pancreas preparations using an organ bath. Exp. Anim., 67, 15-22, 2018.
  • Shitara, A., Shibui, T., Okayama, M., Arakawa, T., Mizoguchi, I., Sakakura, Y., and Takuma, T.: VAMP4 and its cognate SNAREs are required for maintaining the ribbon structure of the Golgi apparatus. J. Oral Biosci., 59, 192-196, 2017
  • Milberg, O., Shitara, A., Ebrahim, S., Masedunskas, A., Tora, M., Tran, D.T., Chen, Y., Conti, M.A., Adelstein, R.S., Ten Hagen, K.G., and Weigert, R.: Concerted actions of distinct nonmuscle myosin II isoforms drive intracellular membrane remodeling in live animals. J. Cell Biol., 216, 1925-1936, 2017
  • Mizukoshi, K., Koyama, N., Hayashi, T., Zheng, L., Matsuura, S. and Kashimata, M.: Shh/Ptch and EGF/ErbB cooperatively regulate branching morphogenesis of fetal mouse submandibular glands. Dev. Biol., 412, 278-287, 2016
  • Satoh, K., Narita, T., Katsumata-Kato, O., Sugiya, H., and Seo, Y.: Involvement of myristoylated alanine-rich C kinase substrate phosphorylation and translocation in cholecystokinin-induced amylase release in rat pancreatic acini. Am. J. Physiol. Gastrointest. Liver Physiol., 310, G399-G409, 2016
  • Sramkova, M., Parente, L., Wigand, T., Aye, M.P., Shitara, A., and Weigert, R.: Polyethylenimine-mediated expression of transgenes in the acinar cells of rats salivary glands in vivo. Front. Cell Dev. Biol., 9;2:74, 2015
  • Hayashi, T., Koyama, N., Mizukoshi, K., Azuma, Y. and Kashimata, M.: Extracellular microRNA in saliva. J. Oral Biosci., 56, 91-96, 2014
  • Satoh, K., Senpuku, H., and Sugiya, H.: Involvement of E2f1 deficiency in salivary gland hypofunction: a review of studies of E2f1-deficient NOD/SCID mice. J. Oral Biosci., 56, 77-80, 2014
  • Satoh, K., Narita, T., Matsuki-Fukushima, M., Okabayashi, K., Yamazaki, F., Arai, T., Ito, T., Senpuku, H., and Sugiya, H.: A novel animal model for dry mouth, E2f1-deficient NOD/SCID mice. J. Oral Biosci., 56, 18-22, 2014
  • Porat-Shliom, N., Chen, Y., Tora, M., Shitara, A., Masedunskas, A., and Weigert, R.: In vivo tissue-wide synchronization of mitochondrial metabolic oscillations. Cell Rep., 23, 514-521, 2014
  • Milberg, O., Tora, M., Shitara, A., Takuma, T., Masedunskas, A., and Weigert, R.: Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy. Methods Mol. Biol., 1174, 407-421, 2014
  • Ito, M., Arakawa, T., Okayama, M., Shitara, A., Mizoguchi, I., and Takuma, T.: Gravity loading induces adenosine triphosphate release and phosphorylation of extracellular signal-regulated kinases in human periodontal ligament cells. J. Investig Clin. Dent., 5, 266-247, 2014
  • Niwa, T., Mizukoshi, K., Azuma, Y., Kashimata, M. and Shibutani, T.: Fundamental study of osteoclast chemotaxis toward chemoattractants expressed in periodontitis. J. Periodont. Res., 48, 773-780, 2013
  • Egashira, Y., Suzuki, Y., Azuma, Y., Takagi, T., Mishiro, K., Sugitani, S., Tsuruma, K., Shimazawa, M., Yoshimura, S., Kashimata, M., Iwama, T. and Hara, H.: The growth factor progranulin attenuates neuronal injury induced by cerebral ischemia-reperfusion through the suppression of neutrophil recruitment. J. Neuroinflammation, 10:105, 2013
  • Satoh, K., Narita, T., Matsuki-Fukushima, M., Okabayashi, K., Ito, T., Senpuku, H., and Sugiya, H.: E2f1-deficient NOD/SCID mice have dry mouth due to a change of acinar/duct structure and the down-regulation of AQP5 in the salivary gland. Pflugers Arch., 465, 271-281, 2013
  • Seo, Y., Satoh, K., Morita, H., Takamata, A., Watanabe, K., Ogino, T., Hasebe, T., and Murakami, M.: Mn-citrate and Mn-HIDA: intermediate-affinity chelates for manganese-enhanced MRI. Contrast Media Mol. Imaging, 8, 140-146, 2013
  • Shitara, A., Shibui, T., Okayama, M., Arakawa, T., Mizoguchi, I., Sakakura, Y., and Takuma, T.: VAMP4 is required to maintain the ribbon structure of the Golgi apparatus. Mol. Cell Biochem., 380, 11-21, 2013
  • Takuma, T., Shitara, A., Arakawa, T., Okayama, M., Mizoguchi, I., and Tajima, Y.: Isoproterenol stimulates transient SNAP23-VAMP2 interaction in rat parotid glands. FEBS Lett., 587, 583-589, 2013
  • Koyama, N., Hayashi, T., Mizukoshi, K., Gresik, E.W. and Kashimata, M.: Extracellular regulated kinase 5 is expressed in fetal mouse submandibular glands and is phosphorylated in response to epidermal growth factor and other ligands of ErbB family of receptors. Develop. Growth Differ., 54, 801-808, 2012
  • Hayashi, T., Koyama, N., Mizukoshi, K. and Kashimata, M.: MicroRNA profiling methods applied to recent studies fetal mouse submandibular gland development. J. Oral Biosci., 54, 169-172, 2012
  • Alkayed, F., Kashimata, M., Koyama, N., Hayashi, T., Tanuma, Y. and Azuma, Y.: P2Y11 purinoceptor mediates the ATP-enhanced chemotactic response of rat neutrophils. J. Pharmacol. Sci., 120, 288-295, 2012
  • Satoh, K., Seo, Y., Matsuo, S., Karabasil, M.R., Matsuki-Fukushima, M., Nakahari, T., and Hosoi K.: Roles of AQP5/AQP5-G103D in carbamylcholine-induced volume decrease and in reduction of the activation energy for water transport by rat parotid acinar cells. Pflugers Arch., 464, 375-359, 2012
  • Okayama M, Shitara A, Arakawa T, Tajima Y, Mizoguchi I, Takuma T.: SNARE proteins are not excessive for the formation of post-Golgi SNARE complexes in HeLa cells. Mol. Cell Biochem., 366, 159-168, 2012
  • Koyama, N., Hayashi, T. and Kashimata, M.: Regulation of branching morphogenesis in fetal mouse submandibular gland by signaling pathway activated by growth factors and a6 integrin. J. Oral Biosci., 53, 298-303, 2011
  • Hayashi, T., Koyama, N., Azuma, Y. and Kashimata, M.: Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro. Dev. Biol., 352, 299-307, 2011
  • Satoh, Ka., Satoh, Ke., Imaizumi, Y., Yokoi, M., Kondoh, T., Murakami, M., and Seo Y.: Magnetic resonance imaging of the temporomandibular joint in the rat compared with low-powered light microscopy. Arch. Oral Biol., 56, 1382-1389, 2011
  • Seo, Y., Satoh, K., Watanabe, K., Morita, H., Takamata, A., Ogino, T., and Murakami, M.: Mn-bicine: a low affinity chelate for manganese ion enhanced MRI. Magn. Reson. Med., 65, 1005-1012, 2011
  • Morita, T., Tanimura, A., Shitara, A., Suzuki, Y., Nezu, A., Takuma, T., and Tojyo, Y.: Expression of functional Stim1-mKO1 in rat submandibular acinar cells by retrograde ductal injection of an adenoviral vector. Arch. Oral Biol., 56, 1356-1365, 2011
  • Ohno, K., Koyama, N., Hayashi, T., Takai, Y., Gresik, E.W. and Kashimata, M.: Regulation of expression of Sprouty isoforms by EGF, FGF7 or FGF10 in fetal mouse submandibular glands. Oral Sci. Int., 7, 47-55, 2010
  • Suda, K., Takahashi, Y., Matsuki-Fukushima, M., and Satoh, K.: Effect of the scent from grapefruit oil on salivary secretion in rats. Int. J. Oral-Med. Sci., 9, 31-35, 2010
  • Guo, M.Y., Satoh, K., Qi, B., Narita, T., Katsumata-Kato, O., Matsuki-Fukushima, M., Fujita-Yoshigaki, J., and Sugiya, H.: Thiol-oxidation reduces the release of amylase induced by β-adrenergic receptor activation in rat parotid acinar cells. Biomed. Res., 31, 293-299, 2010
  • Mitsui, R., Fujita-Yoshigaki, J., Narita, T., Matsuki-Fukushima, M., Satoh, K., Qi, B., Guo, M.Y., Katsumata-Kato, O., and Sugiya, H.: Maintenance of paracellular barrier function by insulin-like growth factor-I in submandibular gland cells. Arch Oral Biol., 55, 963-969, 2010
  • Qi, B., Narita, T., Satoh, K., Guo, M.Y., Katsumata-Kato, O., Murakami, M., Fujita-Yoshigaki, J., and Sugiya, H.: Characteristics of neurokinin A-induced salivary fluid secretion in perfused rat submandibular gland. Arch Oral Biol., 55, 737-744, 2010
  • Hayashi, T., Koyama, N., Gresik, E.W. and Kashimata, M.: Detection of EGF-dependent microRNAs of the fetal mouse submandibular gland at embryonic day 13. J. Med. Invest., 56, 250-252, 2009
  • Koyama, N., Hayashi, T., Gresik, E.W. and Kashimata, M.: Role of a6 integrin subunit in branching morphogenesis of fetal mouse submandibular gland: investigation by mesenchyme-free epithelial culture system. J. Med. Invest., 56, 247-249, 2009
  • Gresik, E.W., Koyama, N., Hayashi, T. and Kashimata, M.: Branching morphogenesis in the fetal mouse submandibular gland is codependent on growth factors and extracellular matrix. J. Med. Invest., 56, 228-223, 2009
  • Sugiya, H. and Satoh, K.: Role of protein kinase C in cAMP-dependent exocytosis in parotid acinar cells. Jpn. Dent. Sci. Rev., 45, 121-126, 2009
  • Shitara, A., Tanimura, A., Sato, A., and Tojyo, Y.: Spontaneous oscillations in intracellular Ca2+ concentration via purinergic receptors elicit transient cell swelling in rat parotid ducts. Am. J. Physiol. Gastrointest. Liver Physiol., 297, G1198-G1205, 2009
  • Satoh, K., Matsuki-Fukushima, M., Qi, B., Guo, M.Y., Narita, T., Fujita-Yoshigaki, J., and Sugiya, H.: Phosphorylation of myristoylated alanine-rich C kinase substrate is involved in the cAMP-dependent amylase release in parotid acinar cells. Am. J. Physiol. Gastrointest. Liver Physiol., 296, G1382-G1390, 2009
  • Matsui-Inohara, H., Uematsu, H., Narita, T., Satoh, K., Yonezawa, H., Kuroda, K., Ito, T., Yoneda, S., Kawarai, T., Sugiya, H., Watanabe, H., and Senpuku H.: E2F-1-deficient NOD/SCID mice developed showing decreased saliva production. Exp. Biol. Med., 234, 1525-1536, 2009
  • Satoh, K., Guo, M.Y., and Sairenji, N.: Involvement of phosphodiesterase 4 in beta-adrenoceptor agonist-induced amylase release in parotid acinar cells. J. Oral Sci., 51, 173-179, 2009
  • Tanimura, A., Morita, T., Nezu, A., Shitara, A., Hashimoto, N., and Tojyo, Y.: Use of Fluorescence Resonance Energy Transfer-based Biosensors for the Quantitative Analysis of Inositol 1,4,5-Trisphosphate Dynamics in Calcium Oscillations. J. Biol. Chem., 284, 8910-8917, 2009
  • Koyama, N., Hayashi, T., Ohno, K., Siu, L., Gresik, E.W. and Kashimata, M.: Signaling pathways activated by epidermal growth factor receptor or fibroblast growth factor receptor differentially regulate branching morphogenesis in fetal mouse submandibular glands. Develop. Growth Differ., 50, 565-576, 2008
  • Mizogami, M., Tsuchiya, H., Takahiro, U., Kashimata, M. and Takakura, K.: Stereospecific interaction of bupivacaine enantiomors with lipid membranes. Reg. Anesth. Pain Med. 33, 304-311, 2008
  • Matsuki-Fukushima, M., Hashimoto, S., Shimono, M., Satoh, K., Fujita-Yoshigaki, J., and Sugiya, H.: Presence and localization of aquaporin-6 in rat parotid acinar cells. Cell Tissue Res., 332, 73-80, 2008
  • Matsuura, S., Koyama, N., Kashimata, M., Hayashi, H. and Kikuta, A.: Temporary accumulation of glycogen in the epithelial cells of the developing mouse submandibular gland. Anat. Sci. Int., 82, 164-174, 2007
  • Azuma, Y., Kosaka, K. and Kashimata M.: Phospholipase D-dependent and -independent p38MAPK activation pathways are required for superoxide production and chemotactic induction, respectively, in rat neutrophils stimulated by fMLP. Eur. J. Pharmacol., 568, 260-268, 2007
  • Qi, B., Fujita-Yoshigaki, J., Michikawa, H., Satoh, K., Katsumata, O., and Sugiya, H.: Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells. Cell Tissue Res., 329, 59-70, 2007
  • Shitara, A., Tanimura, A., Nezu, A., Morita, T., and Tojyo, Y.: Multi-photon microscopic imaging of rat parotid ducts demonstrates cellular heterogeneity in Ca2+ responsiveness. Arch. Oral Biol., 52, 1072-1078, 2007
  • 小山典子、大野健二、松本嗣央、高井良招、柏俣正典:胎仔マウス顎下腺における新規MAPキナーゼErk5の発現と機能的役割、岐阜歯科学会雑誌、33、116-120、2006
  • Azuma, Y. and Kashimata, M.: Role of calcium-calmodulin in superoxide production and chemotaxis in FMLP-stimulated rat neutrophils. Inflam. Regener., 26, 460-466. 2006
  • Sairenji, N., Satoh, K., and Sugiya, H.: Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase in cGMP metabolism in rabbit parotid acinar cells. Biomed. Res., 27, 37-44. 2006
  • Nezu, A., Tanimura, A., Morita, T., Shitara, A., and Tojyo, Y.: A novel fluorescent method employing the FRET-based biosensor "LIBRA" for the identification of ligands of the inositol 1,4,5-trisphosphate receptors. Biochim. Biophys. Acta., 1760, 1274-1280. 2006
  • Tsuchiya, H., Mizogami, M., Kashimata, M. and Takakura, K.: Comparative membrane-fluidizing effects of phenolic agents. Dentistry in Japan, 41, 35-38, 2005
  • Adachi, N., Fujiwara, S., Tanabe, T., Muramatsu, M., Isozaki, N., Kashimata, M. and Nagahara, K.: Statistic elevation of questionnaire about the bad breath to the patients and attendance of Asahi University. J. Gifu Dent. Soc., 32, 16-20, 2005
  • 土屋博紀、溝上真樹、大嶋和之、柏俣正典、高倉 康:構造ならびに液性に依存した局所麻酔薬の膜流動化作用と逆相分配HPLC保持能、薬学雑誌、124、133-136、2004
  • Gresik E.W., Koyama, N., Miyazaki, Y., Siu, L., Hieda, Y. and Kashimata, M.: Regulation of branching morphogenesis in the fetal mouse subamnddibular gland by ErbB signaling system. Anat. Sci. Int., 79, 128, 2004
  • Kashimata, M., Koyama, N. and Gresik, E.W.: Function and related signaling pathway of EGF system (EGF ligand and ErbB family) expressed in fetal mouse submandibular gland. J. Jpn. Salivary Glands, 45, 90-91, 2004
  • Koyama, N., Gresik, E.W. and Kashimata, M.: EGF-stimulated signaling regulates branching morphogenesis of fetal mouse submandibular glands. J. Jpn. Salivary Glands, 45, 89-90, 2003
  • Tokunaga, T., Morshed, S.R.M., Otsuki, S., Takayama, F., Sato, T., Hashimato, K., Yasui, T., Ogawa, S., Kanegae, H., Yokose, Y., Akahane, K., Kashimata, M., Satoh, K. and Sakagami, H.: Effect of antioxdants, oxidants, metals and saliva on cytotoxicity induction by sodium fluoride. Anticancer Res., 23, 3719-3726, 2003
  • Morshed, S.R.M., Tokunaga, T., Kashimata, M. and Sakagami, H.: Effect of antitumor agents on cytotoxicity induction by sodium fluoride. Anticancer Res., 23, 4729-4736, 2003
  • Tokunaga, T., Morshed, S.R.M., Otsuki, S., Takayama, F., Kashimata, M., Nakamura, Y., Nishikawa, H., Yasui, T., Yokose, Y., Akahane, K. and Sakagami, H.: Effect of endodontic agents on cytotoxity induction by sodium fluoride. In Vivo, 17, 583-592, 2003
  • Koyama, N., Kashimata, M. and Gresik, E.W.: EGF-stimulated signaling of PI3K, PLCg1, PKC isozymes regulates branching morphogenesis of fetal mouse submandibular glands. Dev. Dyn., 227, 216-226, 2003
  • 小川了美、柏俣正典、坂上 宏、鐘ヶ江晴秀:マウス歯周組織におけるインテグリン vサブユニットおよびEGF受容体の発現.明海歯学誌、32、126-129、2003
  • Tanimura, A., Shitara, A., Nezu, A., Morita, T., and Tojyo, Y.: Differential Ca2+ responses induced by thrombin and thrombin-receptor agonist peptides in HSY-EA1 cells. Cell Biol. Int., 27, 1017-1023, 2003
  • Tsuchiya, H., Nagayama, M., Tanaka, T., Kashimata, M. and Takeuchi, H.: Membrane-regidifying effects of anti-cancer dietary factors. Biofactors, 16, 45-56, 2002.

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